Análise bacteriológica de mãos, região orofaríngea e equipamentos de proteção individual dos manipuladores de alimentos de um Restaurante Universitário no Rio de Janeiro / Bacteriological analysis of hands, orofaringea region and individual protection equipment of food manipulators of a university restaurant in Rio de Janeiro

Objetivo do trabalho, realizar análise bacteriológica de mãos, região nasal e orofaríngea e dos Equipamentos de Proteção Individual dos manipuladores de alimentos e correlacionar com as ações analisadas ao longo da cadeia produtiva. Pesquisa bacteriológica e observacional realizada no ano de 2016, em Restaurante Universitário/RJ, com 17 manipuladores, para contagem de Staphylococcus coagulase positiva, pesquisa de Salmonella spp. e Número Mais Provável de Escherichia coli totais e análise das ações dos manipuladores durante as atividades laborais. Na pesquisa bacteriológica para mãos e Equipamentos de Proteção Individual, obteve-se 22,4% de resultados positivos para as bactérias pesquisadas, com exceção da pesquisa para Salmonella com ausência em todas as análises; quanto a região nasal e orofaríngea, 35,3% foram positivas para Staphyloccocus spp. Conclui-se que o serviço possui falhas nos controles higiênico sanitários dos manipuladores, com riscos à saúde do consumidor.

Contamination of Carcasses and Utensils in Small Swine Slaughterhouses by Salmonella in the Northwestern Region of the State of Rio de Janeiro, Brazil.

Salmonella is a major foodborne pathogen that constantly threatens food safety in developed countries and underdeveloped countries such as Brazil, where it is responsible for 38% of notified cases of foodborne illness. Swine are one of the main meat-producing species that may asymptomatically carry Salmonella, periodically shedding the bacteria through feces. The state of Rio de Janeiro is not a major producer of swine meat, but small slaughterhouses are operational and produce meat for consumption within the state, although few studies have been conducted in the region. In this context, this study was designed to evaluate Salmonella contamination in carcasses, lymph nodes, feces, utensils, and the environment of three small slaughterhouses in the state of Rio de Janeiro. A total of 344 samples from carcasses, utensils, equipment, water, and the environment were collected from these slaughterhouses in the northwestern region of Rio de Janeiro. Salmonella was isolated from 36 (10.5%) samples: 10 of 48 carcasses, 19 of 96 lymph nodes, 4 of 48 fecal samples, 2 of 6 water samples, and 1 of 6 bleeding knives; 55 isolates were recovered. Serotyping revealed the predominance of Salmonella Typhimurium (20 isolates) followed by Salmonella Abony (10 isolates), Salmonella Give (7 isolates), Salmonella Heidelberg (4 isolates), and Salmonella Infantis (1 isolate). Ten isolates were only partially typeable, with only their O antigen identified, and three isolates had rough, nontypeable colonies. Despite the overall low prevalence of Salmonella in this study, all three slaughterhouses had poor hygienic and sanitary conditions, providing easy routes for carcass and, consequently, meat contamination. Thus, it is imperative to enforce sanitary inspections in these establishments and to apply good manufacture practices to assure the safety of the produced pork.